|
|  |
Year 1
Months 1-3 (Ashlock): Design 800-1,000 pairs of primers/month to amplify introns from unclustered methyl-filtered and high Cot sequences.
Month 4 (Ashlock and Schnable): Generate or acquire clustered methyl-filtered and high Cot sequences.
Month 4 (Ashlock): Begin use of new machine-learning-based primer design tool.
Months 4-12 (Ashlock): Design 800-1,000 pairs of primers/month; beginning in month 4 primers will be designed based on clustered genomic sequences (total primers per year = 10,000)
By Month 12 (Schnable): Map 1,300 genes.
By Month 12 (Buckner): Clone and sequence full-length cDNA clones of 3 genes; analyze DNA sequence polymorphisms in 16 alleles/gene.
By Month 12 (Ashlock & Schnable): Based on PCR results over past
year, evaluate value of new primer design tool. Decide whether to use
new or old design system for future years.
By Month 12 (Buckner & Schnable): Conduct Scientific/Cultural Experience for undergraduates.
Year 2
Months 1-12 (Ashlock): Design 800-1,000 pairs of primers/month (10,000 in Year 2).
By Month 12 (Schnable): Map 1,700 genes.
By Month 12 (Buckner): Clone and sequence full-length cDNA clones of 3 genes; analyze DNA sequence polymorphisms in 16 alleles/gene.
By Month 12 (Buckner & Schnable): Conduct Scientific/Cultural Experience for undergraduates.
Year 3
Months 1-12 (Ashlock): Design 10,000 pairs of primers.
By Month 12 (Schnable): Map 1,500 genes.
By Month 12 (Buckner): Clone and sequence full-length cDNA clones of 3 genes; analyze DNA sequence polymorphisms in 16 alleles/gene.
By Month 12 (Buckner & Schnable): Conduct Scientific/Cultural Experience for undergraduates.
|
|
