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IDP data
A major goal of this project is to genetically map thousands of maize genes and to develop these
genes as genetic markers. The methods used for mapping are described here. In brief, primers designed from maize genes are used to identify polymorphisms between
the inbred lines B73 and Mo17 that can be used to map the corresponding genes. Maize genes are identified via alignments between ESTs and individual GSSs and
assembled GSSs, i.e., MAGIs or via ab initio predicted maize genes. We are detecting polymorphisms
using either agarose gel electrophoresis or Temperature Gradient Capillary Electrophoresis TGCE.
Gene markers mapped on the IBM-94 panel
(Excel file - 2.3 MB)
Gene markers mapped on six F1BC populations (Excel file - 250 KB)
Data Release Policy
On a monthly basis the Genbank or Contig ID #s of new sequences that have
been mapped by the project (and the primer sequences and PCR conditions
used to map these sequences) will be posted to the project web site and
provided to MaizeGDB and Gramene.
This will allow projects that are interested in physically
mapping genetically mapped sequences to initiate experiments prior to the
release of the genetic mapping publication(s). Mapping scores will be provided
to MaizeGDB and Gramene after the acceptance of the relevant peer-reviewed
article(s) or within 3 months of the completion of the project, whichever
comes first. On a quarterly basis gel photos that demonstrate IDPs detected
between B73 and Mo17 will be made available to MaizeGDB.
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